Whether drizzled over porridge or used to treat a sore throat, honey is one of our most cherished food products. In the UK, honey consumption is steadily rising but competition from cheaper imports and barriers to entering the ‘premium’ honey market could stop UK producers from benefitting. A project funded by the STFC Food Network+ (SFN) is helping to address this by applying a pioneering spectroscopic technique for a new use in the honey industry
Honey samples collected by Maria and her team for the study (Image credit: Maria Anastasiadi).
The UK consumes over 40,000 tonnes of honey per year, however most of the honey we see on supermarket shelves is imported from abroad where it is cheaper to produce. But a growing interest in ‘monofloral honeys’ – where the bees collect pollen from a single type of flower – could open up a new market for UK beekeepers. These honeys have characteristic flavours and textures, besides enhanced health benefits including high concentrations of antioxidant compounds. Consequently, monofloral honeys can command premium prices. Heather honey, for instance, can retail for up to £14 for a 340g jar.
But it is currently difficult for beekeepers to enter this lucrative market since there is no quick and easy way for them to prove that their honey is genuinely monofloral. “The only option is to send samples to a dedicated laboratory to analyse the pollen content, which is time consuming and expensive” says bioinformatician Dr Maria Anastasiadi (Cranfield University, UK). Recently, Maria’s work has focused on exploring how spectroscopic technologies (where matter interacts with electromagnetic radiation) can be used within the food industry. “I realised that the honey industry really needs an easy-to-use diagnostic tool that can instantly tell whether a honey is monofloral or multifloral in origin. Through the grant from the SFN, we tested three candidate techniques to do this” she says.
The power of spectroscopy
Maria was particularly interested in Spatially Offset Raman Spectroscopy (SORS), a recently developed technique that shows great promise for a wide range of analytical applications. Raman spectroscopy analyses the composition of a sample by measuring the degree by which monochromatic light is scattered by the sample’s constituent molecules.
Representative photonics spectra for different types of honey: heather (left), borage (middle), multifloral rapeseed (right) (Image credit: Maria Anastasiadi).
In SORS, the light source is offset, allowing it to penetrate deeper without being obscured by the overlying surface material. So far, SORS has been demonstrated capable of detecting explosive materials inside containers and impure pharmaceuticals within sealed blister packs; it is also being investigated for a tool in breast cancer diagnosis. Food industry applications of SORS have so far been limited, and it until now it had never been tested on honey products. During the 2019 SFN Sandpit event, Maria met Professor Pavel Matousek, one of the inventors of the SORS technique at the Science and Technology Facilities Council’s Rutherford Appleton Laboratory. “We were really lucky to meet Pavel, as he is an established leader in this field and agreed to become a co-investigator on this proposal” she says.
To start with, Maria and Pavel collected over fifty different honey samples, including monofloral heather and borage honeys from across the UK and multifloral honeys. Alongside SORS, the group also tested two more established spectroscopic techniques; Attenuated Total Reflection Fourier Transform Infrared and fluorescence spectroscopy. The spectroscopic profiles produced by the different samples were then fed into a machine learning algorithm to train a model to automatically classify the floral origin of unknown samples. “When we introduced samples that the model had not encountered before, we found that both SORS and fluorescence could predict the floral type with over 90% accuracy” says Maria. “What is particularly exciting is that this is a non-invasive technique that doesn’t even need the sample container to be opened.”
Protecting the farmers
With this success, the team wondered whether spectroscopy could help solve another problem – an ongoing battle against adulterated products. Typically, this occurs when pure honey is diluted with cheap high-fructose corn syrup or other sugars and occurs mostly outside the UK. Easy-cost efficient tools to establish authenticity could help British bee farmers safeguard the quality of their product and increase its value.
Maria and her team diluted pure heather honey with known amounts of corn syrup and again used the spectroscopic profiles in a machine learning model. “The prediction models developed using fluorescence and SORS were able to identify adulterated samples with over 80% accuracy” says Maria. “If the model was expanded to include more samples, we believe that this could be enhanced to distinguish adulteration even at very small scales.”
The end goal
With the positive results from this project, Maria is now looking to develop these techniques into a simple diagnostic device for the honey industry. Potentially, this could both help honey producers to authenticate their premium monofloral honeys and give customs officials and honey suppliers a tool to spot fraudulent products in transit. “It is really important that the end-product is something that is easy to use and can instantly give the user an answer they understand. We are now working closely with stakeholders in the UK honey industry and recently organised a webinar to discuss how we can develop a platform for honey testing based on portable sensors.”
The benefits of a network
“I am at an early stage in my academic career, so this has been a valuable opportunity to develop a project of my own. The SFN has been extremely helpful throughout in addressing my questions and offering constant support” Maria says. She is also grateful to the Bee Farmers’ Association who put them in touch with beekeepers across the UK to help them source honey samples. Maria hopes that, in time, the fruits of this project will bring long-term benefits to their industry: “One of the things that really inspired me to do this project was my worry about the decline of honey bees and other pollinators. I hope that the results of this project can help promote British-produced honey and generate more interest in amateur beekeeping” she concludes.
Curious to know more about SORS? Check out our previous blog post ‘Caught in the act’ about an SFN-project that explored whether SORS could help detect adulterated fruit juices.
In our last blog post, we told the story of how the STFC Food Network+ (SFN) didn’t let a global pandemic stop the most recent Sandpit from going ahead by transitioning the whole event online for the first time. Although this gave a very different experience to the usual, in-person format, this clearly didn’t impact the quality of the proposals which resulted from the discussions. Here we feature the winning projects for each theme, which were decided by democratic vote to receive £10K each in funding.
Theme 1: Sustainable production at uncertain times – smart beehive monitoring
Bees and other pollinators play a crucial role in agriculture: approximately 70% of our food crops depend on them. Yet pollinators are suffering from a perfect storm of challenges, including diseases, climate change, air pollution and toxic insecticides. For the SFN Sandpit’s winning theme 1 project, Paulette Elliott (Huduma) and her colleagues will be developing a prototype sensor to monitor honey bee hives in real time. “There are already various sensors on the market, for instance to monitor the hive’s humidity or to count the bees as they enter and leave the hive” Paulette says. “Our aim is to use machine learning and artificial intelligence to analyse the data from these existing sensors to see where there are repeated anomalies or trends that can identify specific problems.” This knowledge will then be applied to build a proof-of-concept sensor for a particular issue. In contrast to existing sensors that currently use Wi-Fi or mobile data technologies, the new sensor will be linked with satellite technology, extending its capabilities further. For instance, earth observation remote sensing information could help beekeepers understand if their hives are in the optimum location, either to avoid air pollution hotspots or to access the bee’s preferred source of pollen.
Besides involving experts across a broad range of fields, the project is also committed to engaging with end-users. As Managing Director of Huduma, Paulette brings significant experience of working with start-ups and SMEs to develop successful business models for emerging technologies, particularly those based on Internet of Things and autonomous systems. Her colleagues and Co-Investigators include high-performance computing specialists, experts in mathematical modelling from STFC Hartree, University of Warwick, The Open University, UKRI-STFC and the company OPTIfarm, which currently provides real-time monitoring systems for poultry farms. “We intend to collaborate with beekeepers’ networks across the country, including both hobbyists and commercial” says Paulette. “Rather than assuming what the most important issues are, we want to work in partnership with them to identify the most critical factors for bee colony health.”
Theme 2: Resilient food supply chains at uncertain times – Better indicators to spot food fraud
Ever since food began being traded as a commodity, fraudsters have tried to make a quick profit at the customer’s expense. Fraud costs the UK food economy approximately £11 billion each year, but this may be only the tip of the iceberg since fraud is frequently underreported. Besides the financial repercussions, this can have serious health consequences, for example by exposing consumers unknowingly to allergens. The Sandpit’s Theme 2 winning project aims to combat this by equipping authorities with more powerful tools to identify cases of food fraud as they happen.
“It’s currently a huge challenge to detect food fraud. Often, agencies only become aware if it causes ill health or if they are alerted by an insider” says project leader Edward Smart (University of Portsmouth). To identify potential indicators of food fraud, the project will bring together a broad range of food-related databases, including import/export databases, global temperature data and commodity prices. This will be combined with a historical database of known incidences of food fraud. Data science experts at the STFC will then use powerful computational methods to hunt for patterns and trends that could have predicted these events. “As an example, if the price of a foodstuff such as oats suddenly decreases in value, this could be a sign that the market is becoming flooded with a poor quality or fraudulent product” says Edward. The ability to link such indicators to fraudulent activities, particularly during shock events such as the coronavirus lockdown, could help policy makers develop strategies to reduce the flow of fraudulent goods, such as more targeted border checks.
“The Sandpit came at an opportune time since my colleague Lisa Jack and I had just completed a project on calculating the true cost of food fraud” says Edward. “At the Sandpit we were introduced to food researchers from the University of Central Lancashire, Brunel University and Fera Science Ltd. Food fraud was a common interest for all parties and as we started talking, it became clear that data science techniques could be a powerful tool in finding more effective indicators of food fraud.
Theme 3: Nutritional security & consumer behaviour at uncertain times - Intelligent Data Analytics to Understand Food Consumer Practice during Food Shocks
“COVID-19 and the associated lockdowns clearly demonstrated how consumer behaviour can ‘shock’ food systems, resulting in essential items becoming scarce” says Laura Wilkinson (Swansea University). “For food systems to be resilient against future shocks- for instance, as a result of further lockdowns, Brexit or climate change – it is vital that decision makers can anticipate how consumers will react.” In their winning project, Laura and her colleagues will look for trends in consumer behaviour using the huge volume of online commentary that the pandemic has generated, which can act as a ‘window’ into why people behaved as they did.
To convert this heterogenous mix of images, videos and text into meaningful information, Laura and her colleagues will use an approach that combines citizen science and intelligent data analytics. “Our first step will be to categorise the data and describe the sentiment of posts: is the person laughing about the shortage of a particular food, or are they genuinely panicked about it?” The project will explore data from various sources, including Twitter, Facebook, YouTube, Instagram and Deliveroo reviews. “Using a citizen science platform (Zooniverse), we will invite members of the public to help us categorise images and text to form a ‘training dataset’. Then we will apply deep learning techniques to this dataset to teach a computer to perform the process automatically” says Laura. The end result will be a preliminary model that will be able to forecast specific events (such as flour running out of stock) on the basis of the text and images that individuals post online (e.g. comments about flour).
Similar to the other winning proposals, the project brings together a diverse range of disciplines, involving psychologists, nutritionists, economists, computer scientists and data analysts. “We also benefit from being able to access STFC’s high-performance computing facilities and experience in running citizen science projects” Laura says. If successful, she hopes that the group will be able to access additional funding to both scale-up the model and engage retailers, so that they can additional information, such as sales data, to better understand the relationship between consumer sentiment and their buying behaviours.
Look out for our future blog posts which will give updates on the progress of the projects funded in our previous Sandpit events and funding calls
Even the most interactive events based on networking and group discussion can be successfully run online – as the recent STFC Food Network+ (SFN) virtual Sandpit demonstrated.
An opportunity within a challenge
The SFN believes that innovation comes when thinkers from different disciplines are supported and encouraged to try new ideas, even if they have no guarantee of success. The SFN’s Sandpit events play an instrumental role in this, by facilitating informal networking followed by focused brainstorming to develop proposals for collaborative projects that explore a new approach to solve a problem. These proposals are pitched to the assembled participants with the winning entries for each theme decided by democratic vote to receive immediate funding. Face to face interaction is integral to these events; hence with coronavirus-related social distancing restrictions in place, it was clear that the Sandpit scheduled for July 2020 couldn’t be run in the usual format. Like so many events across the globe – from international scientific meetings to cultural festivals – the easiest action would have been to cancel the entire Sandpit. But as SFN technical lead Rakesh Nayak explains, the team felt that the situation actually made it more important than ever to run the Sandpit. “We saw an opportunity to fund some really innovative projects addressing the unprecedented situation the food industry is currently in” he says. “Working within uncertainty is such a focus right now, and learning lessons from this situation could help us address future challenges.” This was reflected in the three themes chosen for the event: Sustainable production at uncertain times; Resilient food supply chains at uncertain times; and Nutritional security & consumer behaviour at uncertain times.
Recreating the experience
Having decided to proceed, the team then spent two months exploring a range of different platforms to work out how to best recreate the experience online. “We were really keen to somehow retain the sensation that participants were actually present at an event happening in real time” Rakesh says. For this reason, LearnBrite was trialled to host the introductory seminar since it allowed the organisers to create a virtual 3D environment, with each participant represented by a personal avatar. “This really did help to recreate the experience of being in a physical auditorium, listening to different presenters - the delegates could even ‘walk’ in to the virtual environment and choose the seat they sat in” says Rakesh. For the following discussions however, Zoom was chosen since it had a greater capacity to manage a high volume of interactions and came with the option of organising groups into separate breakout rooms.
Networking in virtual space
Recognising that an online format would make informal networking harder, the SFN team decided to organise participants into groups with shared interests ahead of the event. “The discussions were held on different days for each theme, so that delegates could work on project proposals for as many themes as they wanted to” says Network Coordinator Gareth Crockett. “But for each theme we assigned participants to subgroups to help us focus the discussions.” These groups were based on the areas of interest that delegates had selected on the registration form. “The aim was to focus the discussion, not to restrict ideas” says Rakesh. “Everyone had the option at any point to change to a different subgroup if they felt it would suit them better.” Judging by feedback comments from delegates, it was generally felt that this approach helped to save time, increase efficiency and reduce social awkwardness in approaching potential new collaborators. “The process was very effective in bringing people together with similar passions and concerns, since we could state our interests on the application form. You need some common ground to start a focussed discussion” says participant Laura Wilkinson (Swansea University). The discussions also sparked new collaborations between industry and academia. “During the Sandpit I made new commercial and academic contacts which I consequently introduced to an existing commercial partner" says Geraint Morgan (The Open University). "This partner later told me that one of these contacts was working in an area they envisaged as being critical to their work, potentially saving them thousands in research. It has led to a very exciting new collaboration to extract high value nutrients from what is currently waste vegetable material.”
The real proof of success, however, came the following week when the participants reconvened to pitch their proposals. Stephen Serjeant (the Open University), who acted as an observer, noted that the online format certainly hadn’t compromised the ambition within the proposals: “I felt the pitches themselves were a step up in quality from previous events" he says. "The online format worked well and nearly early every presentation was skilfully timed to fit their slot." Audience feedback also indicated that the process was much more efficient than usual with many appreciating the anonymity of the voting system. Look out for our next blog post, where we will feature the winning projects for each theme.
“The success of this online Sandpit could set a trend where our future physical events are supplemented by online options to increase our reach and allow us to be more responsive to new challenges” says Rakesh. With the pitches demonstrating that online formats don’t necessarily mean a compromise in quality, Rakesh is keen to capitalise on the wider benefits, such as increased access. As Laura notes, “Having the Sandpit as an online event gave an opportunity for people to attend who may not usually be able to, for instance due to caring responsibilities or lack of funding.” Notably, this was the first Sandpit to have a prominent international dimension, including many participants from Asia and Africa. Online events are also considerably more sustainable, since they have reduced carbon footprints and no catering waste.
"It's never been so important to give people the chance to do high-risk, high-return work as the SFN does. The last Sandpit showed that they come up with some wonderfully imaginative approaches, and I expect at least some of these to pay off handsomely." Stephen summarises. Ultimately, we look forward to the day when the SFN can celebrate the achievements of its membership in person. But the current times have shown that it will take more than a global pandemic to stop our work.
A new approach to measure fruit ripening could help increase quality and reduce waste for apple growers
The apple has long been one of the most popular fruits eaten in Britain, with the UK market worth more than £220 million each year. But few people realise that the juicy, ‘fresh’ apple they buy at the supermarket may have been in cold storage for nearly a year. Timing the apple harvest to ensure long-lasting, delicious fruits is a real challenge for farmers – but one that could soon become easier thanks to research supported by the STFC Food Network+.
"Two examples of Braeburn apple samples where multispectral imaging has been tested to see if it can distinguish parts containing starch (stained dark by iodine) from areas where starch had broken down into simple sugars (no staining). As the technology is still in development, the multispectral results are not yet clear for all samples. Photo credit: Deborah Rees and Melina Zempila."
“Most apples are harvested in autumn, but the demand for them lasts all year round” says Deborah Rees, who describes herself as a ‘post-harvest’ biologist at the Natural Resources Institute (University of Greenwich). “This means we have to find a way to slow down the deterioration process and keep them in good quality”. Typically, this involves storing them at a low temperature (between 0.5 and 3°C depending on the variety), in a modified atmosphere with low oxygen levels. But even under these conditions, apples continue to ripen. “The longer you want to store apples, the earlier you have to pick them, so the window for harvesting is very narrow” says Deborah. “Growers currently have very little advance notice of this, so it can be a nightmare to recruit enough labour”. This can ultimately lead to waste if fruit cannot be picked at the right time. To address this, Deborah is researching a new approach to capturing the ripening process as it happens.
As fruits ripen, complex starch molecules are converted into simpler sugars, developing the sweet taste we enjoy. This can be measured using potassium iodide dye (iodine), which turns blue-black in the presence of starch. “Many people remember doing iodine staining at school, for instance staining leaves to look at starch production during photosynthesis or to compare the starch levels in different vegetables” Deborah says. “Apple growers use exactly the same process, typically in the boot of a car, right in the middle of the orchard. It’s messy, time consuming and not particularly safe considering that iodine is a hazardous substance”. Deborah hopes this could be replaced with a method based on multispectral or hyperspectral imaging. These methods use wavelengths beyond the visible light spectrum, including ultra-violet and infra-red light. In the case of hyperspectral imaging, hundreds or even thousands of narrow bands (10-20 nm) can be analysed. “The resulting image depends on what wavelengths are absorbed by the sample, which is affected by the chemical composition” says Deborah. “What we wanted to find out was if we could find a signal signature that depended on the concentration of starch.”
To investigate this, Deborah and her colleagues collaborated with an apple grower in Kent during the harvest season. “We took cut slices from apples at different maturities and compared the iodine-stained slices with images taken using two hyperspectral cameras to identify promising wavelengths” she says. Once the apple harvest was over, they continued to refine the signal processing method using bananas, since these also ripen when starch molecules convert to sugars. “These were a good substitute model for apples since most bananas are imported immature and then artificially ripened using the plant hormone ethylene” says Deborah.
The initial results indicate that hyperspectral imaging shows promise for measuring apple ripening, particularly for wavelengths in the range 460 – 630 nm, 630 – 920 nm. “A particular advantage is that the data give a quantitative readout, whereas iodide staining can only indicate presence or absence of starch” says Deborah. “This could provide a more informative and earlier measurement of starch breakdown.” She uses the analogy of an emptying bathtub, where iodine staining would only tell you when the tub was completely empty. “A quantitative method, on the other hand, can tell you when the tub is half-empty” she says. The next challenge will be to develop a portable multispectral (or hyperspectral if necessary) instrument that could be used directly by farmers on the orchards, without complex training. According to Deborah, some of the UK’s major apple growers have already shown interest. Nigel Kitney of the agricultural and horticultural advice company Hutchinsons says “this is an exciting development which will remove the subjectivity of the starch iodine test enabling growers to harvest the apples at the correct time, improving the product’s consistency for the consumer”.
Besides providing funding, the STFC Food Network+ enabled Deborah to connect with researchers at RAL Space who were experts in spectroscopic methods and data analysis, including planetary scientist Hugh Mortimer and a research scientist, Melina Zempila. “Normally they would work on processing satellite data, so it was an opportunity for them to apply their skills to a very different sector”. In addition, her attendance and presentation about the project at the Network’s annual meeting opened up a discussion about a whole range of other potential technologies and fruits to consider.
Having worked with apples for over 10 years, it is perhaps not surprising that Deborah enjoys eating them too. “My favourite has to be the Russet apple, although the Bramley is of course the best for a good apple crumble” she says. “I seem to be surrounded by apples all the time at the moment. Even when I go out cycling, it’s usually through the Kent apple orchards!”
Satellite technology could help us start to reverse the perilous condition of our soils…
© Photo by Lizzie Sagoo, ADAS
There is a growing crisis underfoot. Our soils – vital to both our ecosystems and agriculture – are degrading at an unprecedented rate, due to factors that include intensive farming, deforestation and pollution. Degraded soils directly affect food security, availability of clean water, global warming mitigation efforts and biodiversity. But it is difficult to know where to begin to address the issue whilst we lack the technology to accurately monitor soil health on a large scale. Thanks to this ambitious new project from the STFC Food Network+, we could soon be using technology from the space sector to shine a light on the state of our soils.
“There is currently a real gap in our knowledge when it comes to monitoring soils on a large scale” says soil scientist Marcelo Galdos (University of Leeds). “Currently, soil analysis typically involves taking a sample at a discrete location and sending it to a laboratory. At a large scale, this would be expensive and highly labour intensive”. Working with collaborators through the STFC Food Network+, Marcelo is developing a proposal for a completely new approach, using remote satellite imaging and biogeochemical modelling. “For this project, we are focusing on estimating soil organic carbon, as this is a proxy for overall soil health” he says. “It indicates the rate at which the soil is being degraded and its ability to produce food or provide ecosystem services, such as purifying water and removing CO2 from the atmosphere”. Consequently, determining soil organic carbon by integrating satellite data, modelling and field measurements could provide an instant map of UK soil health, complementing ‘on the ground’ surveys.
To begin, Marcelo and his colleagues are scoping all the possible ways that satellite data could potentially be used, to produce a shortlist of the most promising approaches. These include using remote sensing to estimate soil temperature, soil moisture levels, land use and land cover, and aboveground biomass. Combining these quantitative measurements with field-scale data could demonstrate how different land uses or crop types affect soil health. “This could even be extended to compare the impact of different soil management systems, such as no-till techniques, cover crops and crop rotations” says Marcelo. If data was collected over a time series, this could even show how the timings between tilling, planting and harvest affect soil quality.
All of this valuable data could ultimately feed into policies to support the UK Government’s 25-year Environment Plan, which aims to incentivise farmers to improve vital ecosystem services, including soils. It could also prove crucial in our ongoing battle against climate change. “Achieving net-zero will only be possible if we both reduce emissions and remove carbon from the atmosphere” Marcelo says. An accurate map of UK soil condition would enable high-carbon areas, such as peatland, to be preserved and also identify regions where soil carbon could be improved through better management techniques.
Clearly, the potential benefits of this project are significant, but it can only succeed with input from a wide range of disciplines – from soil scientists and agronomists, to satellite experts and climate modellers. Indeed, Marcelo had first thought about using satellites to monitor soils several years ago, but the catalyst for the project came when he attended the STFC Food Network+ Sandpit Event ‘Adapting to climate change: climate-smart agriculture’ in March 2019. It was here he met his future collaborators, Lizzie Sagoo (ADAS, an environmental consultancy), Daniel Morton (UK Centre for Ecology and Hydrology) and Martin Hardcastle (University of Hertfordshire). Since then, the network has grown exponentially, resulting in a workshop in September 2019 attended by experts in a wide range of fields. “This had a truly multidisciplinary and even international reach, including participants from Brazil, the US and Norway, besides start-up companies that use satellite data, such as SatSense” says Marcelo. “Talking with so many different people really helped to formulate our ideas”.
Once these techniques have been refined, they could potentially be applied to sensors on drones, opening links to precision agriculture, where inputs (e.g. fertilisers and pesticides) are only applied where and when needed. Ultimately, Marcelo believes major benefits could be realised by this combination of modelling and remote sensing in developing countries, or in his home country of Brazil. “During my early research years, I spent time in Africa researching sustainable agricultural techniques, and I am currently involved in a large project on climate-smart agriculture there. I would really like to apply this approach to support climate change adaptation and mitigation strategies globally” he says.
In the meantime, the challenge of mapping the UK’s soils is enough. And when he’s not at work, Marcelo turns his attention to the soils around his home. “As a soil scientist, perhaps it is not surprising that I am a keen gardener!” he jokes.
For millions of people across the globe, rice is the foundation of their diet. But this particular crop can contain unsafe levels of arsenic: a poisonous mineral that can cause death. One project funded by the STFC Food Network+ is seeking to understand how exactly arsenic accumulates in rice, which could ultimately inform safer production and cooking practices.
Arsenic naturally occurs in underlying rock, particularly in the regions that border the Himalayan mountain range such as India and Bangladesh. This means that arsenic can easily contaminate groundwater in these regions. “Most people in these regions are aware that they shouldn’t drink water contaminated with arsenic” says Manoj Menon, a lecturer of environmental soil science at the University of Sheffield. “But there is a wider issue of arsenic accumulating in the food chain. Rice is especially problematic because it is a very thirsty plant that takes up a lot of water”. Indeed, for typical paddy-field style irrigation systems, it takes an estimated 2,500 litres of water to produce a single kilogram of unmilled rice. Furthermore, for many countries in south Asia, the average daily rice consumption can be as high as 500g-700g per day (pre-cooked weight), compared with just 15g for Europe. Clearly, this issue needs a holistic approach where crop breeding, irrigation schemes and cooking methods are all optimised to reduce arsenic contamination in rice. But before this can start, many fundamental gaps in our understanding need to be answered.
Manoj began this task by asking how arsenic is distributed within the rice grains themselves – does it concentrate in particular regions or is it present throughout? To answer this, he turned to the UK’s national synchrotron Diamond Light Source, based at the Science and Technology Facilities Council’s Rutherford Appleton Laboratory. Diamond works like a giant microscope, but is 10,000 times more powerful than traditional models. It harnesses the power of electrons by accelerating them to near-light speeds, so that they give off light a billion times brighter than the sun. The light is directed into laboratories known as ‘beamlines’, where it is used to study anything from viruses and vaccines to ancient scrolls and jet engines.
“Our samples were longitudinal sections of individual white rice grains, less than a millimetre thick” says Manoj. Using the X-ray beamline, Manoj produced a high-resolution map comparing the distribution of arsenic with other compounds. Crucially, arsenic was mostly concentrated around the outer layers of the grains. The essential nutrient zinc, on the other hand, was present around the embryonic part of the seed. “This fits previous works and also suggests that arsenic levels could be reduced without affecting the abundance of important micronutrients. This could be through refining the polishing process that removes the outer bran layer, or through alternative cooking methods”.
Following this, Manoj investigated how arsenic levels varied across different rice cultivars and genotypes. His range of samples covered 55 different varieties, including both wild rice and supermarket brands. “We looked at brown rice, white rice, long grain, short grain, medium grain, organically produced and non-organically produced” says Manoj. Since it took between eight and nine hours to produce each high-resolution map using the Diamond Light Source, Manoj used classic analytical techniques to allow a faster comparison: Liquid Chromatography and Mass Spectrometry. Reassuringly, the results showed that for most of the samples, the levels of arsenic fell well below the European safety threshold for adults of less than 0.25 milligrams per kilogram. However, many of the samples exceeded the threshold for children, who have a much lower safety limit of 0.1 milligrams per kilogram. “In particular the highest arsenic levels were seen in organic rice samples” says Manoj. The results also confirmed previous studies which found that brown rice has higher arsenic levels than white. But Manoj cautions against avoiding brown rice on this principle: “Brown rice has health benefits not found in white rice, including higher levels of fibre, vitamins and minerals”.
Since these initial results, the project has taken on a momentum of its own. “After this work with the STFC Food Network+, we have secured additional funding from the Global Challenges Research Fund, which allowed us to set up an Arsenic in Rice Research Network (ARRNet)” Manoj says. He is currently using this to investigate how different cooking methods may affect the distribution of arsenic, besides conducting surveys to understand how aware people in India and Bangladesh are of arsenic contamination in rice. A rice field experiment has been planned in India in 2020-21 to optimise irrigation practices. “Our long-term goal is to apply this knowledge in these regions to help people live with arsenic in the environment” Manoj says.
“This initial small grant from the STFC Food Network+ acted as a spark that has really changed my life a lot” he adds. “It is a brilliant initiative to have small pots of money available that are easier for researchers to access than big grants with more competitive and lengthy application processes. This helps to get projects started”. Despite his work, he still enjoys a good plate of rice, and advises that Europeans shouldn’t be too worried about arsenic contamination. “Our message is that it is the total amount of rice you eat that is the main risk factor” he says. “For the average consumption rate of Europeans, arsenic contamination shouldn’t be a problem, although it is perhaps best to restrict how much rice children are given”.
Manoj and his team have had a paper published on this project - Menon et al (2020): Do Arsenic levels in rice pose a health risk to the UK population? Ecotoxicology and Environmental Safety
Across the world, many communities are already experiencing increasing droughts due to climate change. As the global population increases, securing enough supplies of clean, safe freshwater is a critical priority and using our current resources more efficiently needs to be part of this. Since farming is one of the largest consumers of freshwater, reusing waste water within agriculture could have a significant impact, however existing techniques are limited and difficult to apply at scale. But exciting pilot studies funded through the STFC Food Network+ are already bearing fruit – quite literally – in finding an alternative approach.
“The novel aspect of this project is that we are using an established technique for a purpose it has never been used for before - to purify waste water” says project lead Devendra Saroj, Head of the Centre for Environmental Health and Engineering at the University of Surrey. The main issue with using waste water from industrial sources within agriculture is the presence of contaminants which can then accumulate in plants and seeds. Devendra’s approach is based on treating waste water with pulses of electrons that will react with organic compounds and instantly degrade them. Similar electron beams are already used widely in other applications, such as the security and health sectors.
“Ultimately, using electron beams could purify wastewater to a very high standard within minutes” Devendra says. This is in stark contrast to existing membrane-based purification methods which can take several hours. His initial results on wastewater samples from textiles and mixed industrial uses are already promising, with over 95% of organic compounds being removed. “This is on a par with conventional techniques but much faster” Devendra says. To assess whether this water could safely be used for agricultural purposes, the team have been testing the purified water on plants (such as lettuce and beans) grown in petri dishes. Reassuringly, the results showed little difference between tap water and the purified wastewater, with the seedlings appearing completely healthy with no growth defects. “We are now talking with companies who specialise in hydroponic growing systems, to test this water on vegetables grown commercially” says Devendra. “Since these are closed agricultural systems, these could potentially be coupled to places where waste water is generated”. Another long-term goal is to use wastewater from actual farms, for instance to purify water containing run-off from organic fertilisers.
But the immediate issue is to scale-up the process, since the current bench-size prototype model is only capable of handling sizes up to a litre. This may require a fundamental shift in production processes as Devendra explains: “Most electron beam applications are for scientific uses in laboratory settings. Manufacturers will have to adapt the instruments if they are to be used mainstream”. With so many factors to consider, including cost, ease of use and electron beam concentration, these proof-of-concept results will play a key role in convincing manufacturers to take up the challenge.
For Devendra, the project illustrates perfectly the STFC Food Network’s goal of catalysing new ideas through bringing different disciplines together. “I would encourage researchers to participate in the network, even if they don’t see an immediate connection to their work” he says. It was through one of the Food Network’s Sandpit events, for instance, that he met physicists who develop accelerators that create electron beams. “They hadn’t thought of using this technology in an application like water recycling but with my environmental background I saw the opportunity” Devendra says. “I enjoy actively participating in the STFC Food Network because it allows me to bring my skills and contribute to areas that I don’t have specific expertise in, such as climate change. Bringing different disciplines together allows problems to be addressed from different angles”.
With particular thanks to Dr Donna Pittaway at STFC Daresbury Laboratory
Specially-equipped drones could soon help small holder farmers control one of the deadliest killers of coffee plants.
Coffee is one of the most traded agricultural commodities and has become a lifeline for many developing regions in the world. This includes the Chiang Mai region in Thailand, where the government is on a mission to become the ‘coffee capital of South-East Asia’, focusing on the premium Arabica varieties. But these ambitions could be jeopardised by a parasitic fungus. Coffee leaf rust is a global disease which attacks the leaves of coffee plants, ultimately decimating the coffee bean harvest. The fungal spores spread easily via wind and rain, meaning that a single outbreak can wipe out plantations over entire regions. Sri Lanka, for instance, was once exclusively planted with coffee until a coffee leaf rust epidemic in 1892 destroyed all of the trees, prompting the farmers to plant tea instead. With fungicides being prohibitively expensive and not an option for organically-certified farms, the only way smallholder farmers can control the parasite is to remove infected plants as soon as possible. Yet even this is currently a challenge – although this STFC-funded project could soon make the process much easier.
“It’s very difficult for these farmers to monitor coffee leaf rust, because the plantations are often on the sides of rugged mountains and intercropped with different species: very different from the neat rows we have on UK farms” explains Anthony Brown (Durham University). He first became aware of the problem of coffee leaf rust when he was introduced via the STFC Food Network+ to Oliver Windram, a researcher of remote-sensing technologies for crop plants (Imperial College, London). Oliver’s earlier research in plant pathology had uncovered a real need within agriculture for methods that could quantitatively measure plant disease in the field. This led him to develop remote-sensing technologies for measuring disease on wheat and broccoli, using machine learning to detect and quantify infection. Together, Oliver and Anthony proposed an image-based detection system using drones that could easily fly over the hillsides, allowing infected plants to be identified much more quickly. This draws on Anthony’s expertise in data analytics and unmanned aerial vehicles, and is based on recording spectral data. Their method measures the wavelengths of light reflected off the surface of the plant leaves and records how the intensity of light varies as a function of frequency. The result is a ‘heatmap’ where a large difference in signal intensity indicates the presence of coffee leaf rust.
The project began with field visits to coffee plantations in Thailand to test which wavelengths could most accurately discriminate between healthy and infected plants. This in itself proved an experience: “It was the first time I realised that coffee berries are actually red!” Anthony jokes. The team walked among the plants with spectroscopes to compare the signatures of reflected light from uninfected plants and those with varying degrees of coffee leaf rust. Unfortunately, the optimum wavelengths all fell within a range not covered by commercially-available cameras. “When we tried using off-the-shelf cameras, the images were just not sensitive enough to detect coffee leaf rust. But it is important that this solution is affordable for farmers, who are the ultimate end-users” says Anthony. For this reason, they are now engaging with industrial partners to develop a bespoke filter that would enable a commercial camera to read these wavelengths. Anthony and Oliver hope that by January 2020 the prototype will be ready to undertake proof-of-concept flights over plantations in Thailand.
Focusing on the end-user and engaging them throughout the planning stages also made the team realise that there was another potential application for this technique. “Farmers are really keen to know exactly what varieties of Arabica coffee they have, since pure products can command better prices. But there are very few records for these areas” Anthony says. He is confident, however, that spectral imaging could distinguish between them, potentially opening up new markets for small holders. “Interacting with end-users to learn what is important for them – rather than just assuming what they need - has been one of the most rewarding aspects of this project” he says. Nevertheless, interest in this work has also had a “snowball” effect in opening up a plethora of new research opportunities for him. “Through my work for the STFC Food Network+, I have now been invited to collaborate on projects using remote sensing to understand global warming impacts on trees across Europe and Africa, besides mangrove forests in South America”. The experience has certainly made him keen to be involved with more interdisciplinary projects that combine different skill sets to create novel solutions, and credits the STFC Food Network+ for encouraging these opportunities as part of its fundamental strategy.
“Applying your expertise from one field to another can take you out of your comfort zone, but also be some of the most impactful work that you do” he concludes.
From planets to pig sties… applying technology from space missions could help us control one of the UK’s worst air pollutants
Air pollution has been described as one of the UK’s most severe public health challenges and also damages natural environments. One of the most concerning pollutants is ammonia: besides forming smog and particulate matter that can cause cardiovascular and respiratory disease, it also deposits excess nitrogen in habitats, which reduces biodiversity. Most (88%) of ammonia emissions originate from agriculture, particularly from manure and inorganic fertilisers. Worryingly, unlike other air pollutants such as nitrogen oxides, ammonia emissions are actually increasing and are consequently a key component of the UK Government’s 2019 Clean Air Strategy. But whilst government, industry and researchers agree this trend must be reversed, they face a key obstacle in doing so: measuring ammonia emissions remains no easy task indeed.
“It is really important that we are able to accurately measure ammonia emissions from agriculture” says Daniel Gerber, of STFC Rutherford Appleton Laboratory. “At the moment, most UK studies use wind tunnels to measure ammonia emissions: these are typically 2 metres long and funnel air through a liquid acid, which is analysed later in a laboratory. Although this does work, it is slow and requires significant person power”. A physicist by training, Daniel’s work usually involves equipping space missions with highly sensitive instruments to detect radiation in outer space, rather than more ‘down to earth problems’. Indeed, Daniel wasn’t even aware of how severe the problem of ammonia emissions in the UK was until his group leader Brian Ellison (STFC RAL Space) attended a STFC Food Network+ Sandpit event in March 2018. Here he was introduced to Lizzie Sagoo from ADAS, an agricultural and environmental consultancy, who asked if his methods could be applied to measure ammonia emissions on the ground.
This presented a stimulating challenge for Daniel and his colleagues: “Although our technology can detect ammonia in outer space, measuring it from earth is a completely different scenario. Since space is a vacuum, it is a benign environment for making measurements but in earth’s atmosphere you have other air particles, winds and temperature variations to contend with. We weren’t sure if it could work”. Brian and Lizzie submitted a proposal for an STFC Food Network+ Scoping Grant, to fund a project that would investigate if remote measuring of ammonia emissions was theoretically possible. This was ultimately successful and, starting at the very beginning, the team first worked out which, if any, radio frequencies could be used for detecting ammonia. Every molecule emits tiny radiowave signals, in the form of electromagnetic waves produced by charged particles within the atoms. The frequency of these waves is characteristic of the molecule’s structure; hence a specific gas can be measured by detecting the strength of its characteristic frequency signature. However, these signals can be weakened by other gases in the atmosphere, especially water vapour. This presents a compromise as Daniel explains: “Low frequency signals are less attenuated in the atmosphere but they also tend to be weaker to begin with, so are harder to detect”. Nevertheless, using models of the atmosphere and simulations, he and his colleagues identified a handful of signals that could be potentially viable. These results enabled them to secure a STFC Proof-of-Concept grant to start designing a prototype instrument.
“We envisage that the instrument will be about the size of a small fridge, that could be towed into a field on a trailer or mounted on a wall” Daniel says. Crucially, it would be capable of measuring in real time, allowing it to detect any sudden surges in emissions which could then be investigated. A portable, easy-to-use instrument would also enable a much denser data map of ammonia emissions across the UK. This could ultimately help farmers make informed choices on when and how to spread manure and to assess whether current storage facilities adequately restrict emissions.
These potential gains aptly illustrate the STFC Food Network+’s commitment to support projects that can deliver measurable impact towards a sustainable agricultural sector. “Without the STFC Food Network+, we would not have realised the value of an instrument to measure ammonia on the ground” Daniel says. He also stresses how important it was to interact with real people in order to truly understand what was needed. “Our conversations made us realise that ease of use and operation were more critical than using our space heritage to develop a supremely sensitive instrument. We were also able to visit a farm and see the conditions where it would be used: with dust in the air and mud everywhere, it was clear that this instrument couldn’t only be suited to a clean laboratory environment!”
Besides the cognitive challenge, Daniel has enjoyed the rich experiences the project has brought. “I normally spend all day in clean offices, then suddenly I was pulling on wellies and wading through manure on farms…” he says. Despite the muck, he is keen to become involved in more interdisciplinary work. “This project has made me wonder how many other potential applications that we are not aware of could benefit from our technology… the challenge now is to connect with and meet those users.” After all, you can only begin to try and solve a problem once you know about it. It sums up the STFC Food Network+‘s ethos perfectly: bringing together those with a need with those that have a potential solution.
What conditions create that ‘perfect storm’ where food prices escalate wildly, leading to riots and unrest? Risk analyst Aled Jones (Anglia Ruskin University) is applying techniques he originally used to study the origins of the universe to answer that question – and help protect our food systems from the effects of climate change.
“Making our food systems resilient to climate change is not just about producing enough food, as past events have shown. There have been cases where a small food production shock has had a high effect on food availability, and others where a massive physical disruption had no impact on food accessibility. It all depends on market forces and how governments handle the situation” says Aled. As director of the Global Sustainability Institute, he is applying mathematical data processing techniques to understand how social systems interact with agricultural production to ultimately determine food accessibility. One may presume that the most important factor is our ability to produce food in the first place, but Aled argues that price is the dominant issue. “Price has a greater effect on food access than physical access, since it determines who can actually afford food. But we are really bad at knowing what influences price when there are so many factors, such as markets, speculation and currency values”.
With price being so critical, it is essential to know how this responds to disruptions in food production caused by increasingly frequent extreme weather events (so-called ‘food shocks’). To investigate this, Aled collaborates with the marketing and financial sectors, and, through the foreign office, multinational retail consortia and agrochemical companies. “Insurance companies are often ahead of academia in the sense that they have a lot of data about the conditions that surround food production shocks” he says. The traditional methods used to explore how these different sectors respond to food shocks include interviewing and ‘war game’ scenarios: “Essentially this involves locking representatives from markets and government in a room for a day and seeing how they respond to a hypothetical extreme yet plausible event that affects about 10% of global food production” says Aled.
However, these methods are highly subjective and don’t always correspond with real-world situations. Searching for a more rigorous approach, Aled remembered his days as a PhD cosmology student, where he used image processing techniques to study telescope data on the cosmic microwave background: the oldest electromagnetic radiation in the universe. “I realised that those techniques of categorising information could provide new insights into food price data – uncovering new patterns and causal components”. Through the STFC Food Network+, he formed partnerships with the Department of Applied Maths and Theoretical Physics at the University of Cambridge and the UK Met Office. The aim was to review a range of analytical techniques for their ability to automatically detect food price shocks within datasets. “It’s really the same thing I did in my PhD: applying maths to clean up data and search for hidden patterns within the signals.” Besides tools from the finance and insurance sectors, these techniques include those used to detect earthquakes and exploding supernova stars. As Aled explains: “Normally people look at when a production shock or food riot has happened and then try to work out what it caused or what caused it. But this can miss cases where similar conditions led to different outcomes. Our aim is to find a technique that can objectively identify when a price shock has happened, what the characteristics of a price shock are and a way of categorising them.”. Having identified a number of promising algorithms, the group are now applying these to past historic events.
Ultimately, understanding the conditions and market behaviours that combine to cause food scarcity will allow governments to be proactive and set in place protective strategies. “We hope these techniques can be used to develop hypothetical future scenarios so that governments, businesses and the insurance sector can ‘stress-test’ their plans to manage food shocks” Aled says. He also hopes that it could help develop policies that link up sustainability plans at regional, national and international levels. “Local food systems minimise environmental damage and carbon footprints, but if one is lost due to drought or a flood, you need links to the global system to survive. And when conditions are good, local systems can give their surplus to provide resilience elsewhere” he explains.
Aled credits the STFC Food Network+ with giving him the time and space to try a truly novel idea. “This project has allowed us to take a more objective approach than traditional methods. By looking at historic price events and categorising them, we can then work with partners who explore the impact of weather on food production or the impact of prices on people, to better inform automatic models based on those behaviours.” Meanwhile, whilst this work occupies most of his time, Aled’s original love of astronomy has been rekindled through his young sons’ interests. “I now know more names of moons and dwarf planets than I did when I was actually studying astronomy!” he jokes. It just goes to show how creative thinking and interdisciplinary partnerships can generate truly novel solutions to improve our food systems.